antibodies mouse anti dc sign monoclonal antibody mab 507  (R&D Systems)

Journal: BMC Immunology

Article Title: BMP-6 inhibits growth of mature human B cells; induction of Smad phosphorylation and upregulation of Id1

doi: 10.1186/1471-2172-6-9

Figure Lengend Snippet: BMP-6 inhibits proliferation of human B cells . A) CD19 + B cells were isolated from peripheral blood and stimulated in triplicates with anti-IgM (37.5 μg/ml) or anti-IgM and CD40 ligand (CD40L; 10 ng/ml) in the presence or absence of BMP-6 for 72 hours. DNA synthesis was measured by [3H]-thymidine incorporation for the last 18 hours. Data are given as relative proliferation obtained by normalizing the mean counts per minute (cpm) for each stimulation to the mean cpm obtained for anti-IgM stimulated cells ± SEM. (mean cpm = 25 352 for anti-IgM stimulated cells, *p ≤ 0.0002 (n = 8), **p ≤ 0.023 (n = 6). B) Dose dependent inhibition of BMP-6 of anti-IgM induced DNA-synthesis of CD19 + B cells (relative proliferation ± SEM, n = 3) and C) the Burkitt lymphoma cell line Ramos (relative proliferation ± SEM, n = 3). Ramos cells were cultured for 72 hours and [3H]-thymidine were added for the last 4 hours. D) Noggin (5 μg/ml) and BMP-6 (0,25 or 1 μg/ml) were preincubated for 1 h at 37°C and then added to the CD19 + B cells in the presence of anti-IgM(37.5 μg/ml). Cells were cultured for 72 h and DNA synthesis was measured by 3 H-thymidine incorporation. One representative of three separate experiments is shown (mean cpm ± SD of triplicates). E) Highly purified CD19 + CD27 - or CD19 + CD27 + cells were obtained by cell sorting of CD19 + cells and stimulated with anti-IgM in the presence or absence of BMP-6, as indicated for 72 hours. DNA synthesis was measuredby [3H]-thymidine incorporation. Data are given as relative proliferation obtained by normalizing the mean cpm for each stimulation to the mean cpm obtained for anti-IgM stimulated cells (mean cpm = 18 221 for CD19 + CD27 - naïve B cells, mean cpm = 8 930 for CD19 + CD27 + memory B cells, n = 5; * p ≤ 0,004, **p ≤ 0.001).

Article Snippet: Detection of the BMP-6-protein has been tried with the following antibodies: goat polyclonal anti-BMP-6 (Santa Cruz, San Diego, CA, USA), monoclonal mouse anti-BMP-6 and polyclonal goat anti-BMP-6 from R & D Systems (Abingdon, UK), and mouse monoclonal anti-BMP-6 (Chemicon International Inc, Temecula, CA, USA).

Techniques: Isolation, DNA Synthesis, Inhibition, Cell Culture, Purification, FACS

Journal: BMC Immunology

Article Title: BMP-6 inhibits growth of mature human B cells; induction of Smad phosphorylation and upregulation of Id1

doi: 10.1186/1471-2172-6-9

Figure Lengend Snippet: Ramos cells were cultured in the presence or absense of BMP-6 (1 μg/ml) for 48 h before analysis of cell death by PI staining. Data are shown as mean percentage PI + cells (± SEM, n = 3, p ≤ 0,001).

Article Snippet: Detection of the BMP-6-protein has been tried with the following antibodies: goat polyclonal anti-BMP-6 (Santa Cruz, San Diego, CA, USA), monoclonal mouse anti-BMP-6 and polyclonal goat anti-BMP-6 from R & D Systems (Abingdon, UK), and mouse monoclonal anti-BMP-6 (Chemicon International Inc, Temecula, CA, USA).

Techniques: Cell Culture, Staining

Journal: BMC Immunology

Article Title: BMP-6 inhibits growth of mature human B cells; induction of Smad phosphorylation and upregulation of Id1

doi: 10.1186/1471-2172-6-9

Figure Lengend Snippet: BMP-6 induces cell death in B cells . CD19 + CD27 - naïve B cells or CD19 + CD27 + memory B cells were cultured for 48 h with BMP-6 (1 μg/ml) with or without anti-IgM (37.5 μg/ml). Cell death (PI + cells) was then measured by flow cytometry analysis. Data are shown as mean percentage PI + cells from five independent donors (± SEM; p ≤ 0,003).

Article Snippet: Detection of the BMP-6-protein has been tried with the following antibodies: goat polyclonal anti-BMP-6 (Santa Cruz, San Diego, CA, USA), monoclonal mouse anti-BMP-6 and polyclonal goat anti-BMP-6 from R & D Systems (Abingdon, UK), and mouse monoclonal anti-BMP-6 (Chemicon International Inc, Temecula, CA, USA).

Techniques: Cell Culture, Flow Cytometry

Journal: BMC Immunology

Article Title: BMP-6 inhibits growth of mature human B cells; induction of Smad phosphorylation and upregulation of Id1

doi: 10.1186/1471-2172-6-9

Figure Lengend Snippet: BMP-6-induced phosphorylation of Smad1/5/8 in CD19 + B cells and Ramos, but not in HL60 cells . CD19 + B cells, or the cell lines Ramos or HL60

Article Snippet: Detection of the BMP-6-protein has been tried with the following antibodies: goat polyclonal anti-BMP-6 (Santa Cruz, San Diego, CA, USA), monoclonal mouse anti-BMP-6 and polyclonal goat anti-BMP-6 from R & D Systems (Abingdon, UK), and mouse monoclonal anti-BMP-6 (Chemicon International Inc, Temecula, CA, USA).

Techniques:

Journal: BMC Immunology

Article Title: BMP-6 inhibits growth of mature human B cells; induction of Smad phosphorylation and upregulation of Id1

doi: 10.1186/1471-2172-6-9

Figure Lengend Snippet: were cultured in X-vivo 15 over night before treatment with BMP-6 for 30 minutes, or for the indicated time points before total cell lysates were prepared. The amount of phosphorylated Smad 1/5/8 was determined by western-blot analysis. The membranes were reprobed for Smad1. One representative experiment of three is shown.

Article Snippet: Detection of the BMP-6-protein has been tried with the following antibodies: goat polyclonal anti-BMP-6 (Santa Cruz, San Diego, CA, USA), monoclonal mouse anti-BMP-6 and polyclonal goat anti-BMP-6 from R & D Systems (Abingdon, UK), and mouse monoclonal anti-BMP-6 (Chemicon International Inc, Temecula, CA, USA).

Techniques: Cell Culture, Western Blot

Journal: BMC Immunology

Article Title: BMP-6 inhibits growth of mature human B cells; induction of Smad phosphorylation and upregulation of Id1

doi: 10.1186/1471-2172-6-9

Figure Lengend Snippet: BMP-6 induced upregulation of Id1 at the mRNA and protein level . CD19 + B cells were cultured in X-vivo 15 over night before treatment with BMP-6 for the indicated time points. Total RNA was extracted and Id1 , Id2 or Id3 -expression was analysed by real-time RT-PCR; values are normalised to the expression level of PGK1 mRNA and expressed as relative quantification (2 -average ΔΔC T – relative to Id1 , Id2 or Id3 expression in the cell-line Ramos). One representative of three independent experiments is shown.

Article Snippet: Detection of the BMP-6-protein has been tried with the following antibodies: goat polyclonal anti-BMP-6 (Santa Cruz, San Diego, CA, USA), monoclonal mouse anti-BMP-6 and polyclonal goat anti-BMP-6 from R & D Systems (Abingdon, UK), and mouse monoclonal anti-BMP-6 (Chemicon International Inc, Temecula, CA, USA).

Techniques: Cell Culture, Expressing, Quantitative RT-PCR

Journal: BMC Immunology

Article Title: BMP-6 inhibits growth of mature human B cells; induction of Smad phosphorylation and upregulation of Id1

doi: 10.1186/1471-2172-6-9

Figure Lengend Snippet: Id protein expression was determined by western-blot analysis. CD19 + B cells were cultured in X-vivo 15 over night before treatment with BMP-6 for the indicated time points and cell lysates were prepared. One representative experiment of three is shown. HeLa cells were used as a positive control for Id1 protein detection.

Article Snippet: Detection of the BMP-6-protein has been tried with the following antibodies: goat polyclonal anti-BMP-6 (Santa Cruz, San Diego, CA, USA), monoclonal mouse anti-BMP-6 and polyclonal goat anti-BMP-6 from R & D Systems (Abingdon, UK), and mouse monoclonal anti-BMP-6 (Chemicon International Inc, Temecula, CA, USA).

Techniques: Expressing, Western Blot, Cell Culture, Positive Control

Journal: BMC Immunology

Article Title: BMP-6 inhibits growth of mature human B cells; induction of Smad phosphorylation and upregulation of Id1

doi: 10.1186/1471-2172-6-9

Figure Lengend Snippet: Anti-IgM rapidly upregulates BMP-6 mRNA expression in human B-cells . CD19-positive B cells were cultured in X-vivo 15 over night and stimulated with anti-IgM for the indicated time periods, before total RNA was extracted. The expression of BMP-6 mRNA by realtime RT-PCR; values are normalised to the expression level of PGK1 mRNA and expressed as relative quantification (2 -average ΔΔC T – relative to BMP-6 expression in the cell-line Ramos. One representative of five independent experiments is shown.

Article Snippet: Detection of the BMP-6-protein has been tried with the following antibodies: goat polyclonal anti-BMP-6 (Santa Cruz, San Diego, CA, USA), monoclonal mouse anti-BMP-6 and polyclonal goat anti-BMP-6 from R & D Systems (Abingdon, UK), and mouse monoclonal anti-BMP-6 (Chemicon International Inc, Temecula, CA, USA).

Techniques: Expressing, Cell Culture, Reverse Transcription Polymerase Chain Reaction

Journal: BMC Immunology

Article Title: BMP-6 inhibits growth of mature human B cells; induction of Smad phosphorylation and upregulation of Id1

doi: 10.1186/1471-2172-6-9

Figure Lengend Snippet: Fetal calf serum and human AB serum upregulates BMP-6 mRNA expression in human B-cells . CD19-positive B cells were cultured in X-vivo 15 over night and with fetal calf serum or human AB serum at the indicated dilutions for four hours, before total RNA was extracted. The expression of BMP-6 mRNA by realtime RT-PCR; values are normalised to the expression level of PGK1 mRNA and expressed as relative quantification (2 -average ΔΔC T – relative to BMP-6 expression in the cell-line Ramos. One representative of three independent experiments is shown.

Article Snippet: Detection of the BMP-6-protein has been tried with the following antibodies: goat polyclonal anti-BMP-6 (Santa Cruz, San Diego, CA, USA), monoclonal mouse anti-BMP-6 and polyclonal goat anti-BMP-6 from R & D Systems (Abingdon, UK), and mouse monoclonal anti-BMP-6 (Chemicon International Inc, Temecula, CA, USA).

Techniques: Expressing, Cell Culture, Reverse Transcription Polymerase Chain Reaction

Journal: BMC Immunology

Article Title: BMP-6 inhibits growth of mature human B cells; induction of Smad phosphorylation and upregulation of Id1

doi: 10.1186/1471-2172-6-9

Figure Lengend Snippet: BMP-6 inhibits proliferation of human B cells . A) CD19 + B cells were isolated from peripheral blood and stimulated in triplicates with anti-IgM (37.5 μg/ml) or anti-IgM and CD40 ligand (CD40L; 10 ng/ml) in the presence or absence of BMP-6 for 72 hours. DNA synthesis was measured by [3H]-thymidine incorporation for the last 18 hours. Data are given as relative proliferation obtained by normalizing the mean counts per minute (cpm) for each stimulation to the mean cpm obtained for anti-IgM stimulated cells ± SEM. (mean cpm = 25 352 for anti-IgM stimulated cells, *p ≤ 0.0002 (n = 8), **p ≤ 0.023 (n = 6). B) Dose dependent inhibition of BMP-6 of anti-IgM induced DNA-synthesis of CD19 + B cells (relative proliferation ± SEM, n = 3) and C) the Burkitt lymphoma cell line Ramos (relative proliferation ± SEM, n = 3). Ramos cells were cultured for 72 hours and [3H]-thymidine were added for the last 4 hours. D) Noggin (5 μg/ml) and BMP-6 (0,25 or 1 μg/ml) were preincubated for 1 h at 37°C and then added to the CD19 + B cells in the presence of anti-IgM(37.5 μg/ml). Cells were cultured for 72 h and DNA synthesis was measured by 3 H-thymidine incorporation. One representative of three separate experiments is shown (mean cpm ± SD of triplicates). E) Highly purified CD19 + CD27 - or CD19 + CD27 + cells were obtained by cell sorting of CD19 + cells and stimulated with anti-IgM in the presence or absence of BMP-6, as indicated for 72 hours. DNA synthesis was measuredby [3H]-thymidine incorporation. Data are given as relative proliferation obtained by normalizing the mean cpm for each stimulation to the mean cpm obtained for anti-IgM stimulated cells (mean cpm = 18 221 for CD19 + CD27 - naïve B cells, mean cpm = 8 930 for CD19 + CD27 + memory B cells, n = 5; * p ≤ 0,004, **p ≤ 0.001).

Article Snippet: Detection of the BMP-6-protein has been tried with the following antibodies: goat polyclonal anti-BMP-6 (Santa Cruz, San Diego, CA, USA), monoclonal mouse anti-BMP-6 and polyclonal goat anti-BMP-6 from R & D Systems (Abingdon, UK), and mouse monoclonal anti-BMP-6 (Chemicon International Inc, Temecula, CA, USA).

Techniques: Isolation, DNA Synthesis, Inhibition, Cell Culture, Purification, FACS

Journal: BMC Immunology

Article Title: BMP-6 inhibits growth of mature human B cells; induction of Smad phosphorylation and upregulation of Id1

doi: 10.1186/1471-2172-6-9

Figure Lengend Snippet: Ramos cells were cultured in the presence or absense of BMP-6 (1 μg/ml) for 48 h before analysis of cell death by PI staining. Data are shown as mean percentage PI + cells (± SEM, n = 3, p ≤ 0,001).

Article Snippet: Detection of the BMP-6-protein has been tried with the following antibodies: goat polyclonal anti-BMP-6 (Santa Cruz, San Diego, CA, USA), monoclonal mouse anti-BMP-6 and polyclonal goat anti-BMP-6 from R & D Systems (Abingdon, UK), and mouse monoclonal anti-BMP-6 (Chemicon International Inc, Temecula, CA, USA).

Techniques: Cell Culture, Staining

Journal: BMC Immunology

Article Title: BMP-6 inhibits growth of mature human B cells; induction of Smad phosphorylation and upregulation of Id1

doi: 10.1186/1471-2172-6-9

Figure Lengend Snippet: BMP-6 induces cell death in B cells . CD19 + CD27 - naïve B cells or CD19 + CD27 + memory B cells were cultured for 48 h with BMP-6 (1 μg/ml) with or without anti-IgM (37.5 μg/ml). Cell death (PI + cells) was then measured by flow cytometry analysis. Data are shown as mean percentage PI + cells from five independent donors (± SEM; p ≤ 0,003).

Article Snippet: Detection of the BMP-6-protein has been tried with the following antibodies: goat polyclonal anti-BMP-6 (Santa Cruz, San Diego, CA, USA), monoclonal mouse anti-BMP-6 and polyclonal goat anti-BMP-6 from R & D Systems (Abingdon, UK), and mouse monoclonal anti-BMP-6 (Chemicon International Inc, Temecula, CA, USA).

Techniques: Cell Culture, Flow Cytometry

Journal: BMC Immunology

Article Title: BMP-6 inhibits growth of mature human B cells; induction of Smad phosphorylation and upregulation of Id1

doi: 10.1186/1471-2172-6-9

Figure Lengend Snippet: BMP-6-induced phosphorylation of Smad1/5/8 in CD19 + B cells and Ramos, but not in HL60 cells . CD19 + B cells, or the cell lines Ramos or HL60

Article Snippet: Detection of the BMP-6-protein has been tried with the following antibodies: goat polyclonal anti-BMP-6 (Santa Cruz, San Diego, CA, USA), monoclonal mouse anti-BMP-6 and polyclonal goat anti-BMP-6 from R & D Systems (Abingdon, UK), and mouse monoclonal anti-BMP-6 (Chemicon International Inc, Temecula, CA, USA).

Techniques:

Journal: BMC Immunology

Article Title: BMP-6 inhibits growth of mature human B cells; induction of Smad phosphorylation and upregulation of Id1

doi: 10.1186/1471-2172-6-9

Figure Lengend Snippet: were cultured in X-vivo 15 over night before treatment with BMP-6 for 30 minutes, or for the indicated time points before total cell lysates were prepared. The amount of phosphorylated Smad 1/5/8 was determined by western-blot analysis. The membranes were reprobed for Smad1. One representative experiment of three is shown.

Article Snippet: Detection of the BMP-6-protein has been tried with the following antibodies: goat polyclonal anti-BMP-6 (Santa Cruz, San Diego, CA, USA), monoclonal mouse anti-BMP-6 and polyclonal goat anti-BMP-6 from R & D Systems (Abingdon, UK), and mouse monoclonal anti-BMP-6 (Chemicon International Inc, Temecula, CA, USA).

Techniques: Cell Culture, Western Blot

Journal: BMC Immunology

Article Title: BMP-6 inhibits growth of mature human B cells; induction of Smad phosphorylation and upregulation of Id1

doi: 10.1186/1471-2172-6-9

Figure Lengend Snippet: BMP-6 induced upregulation of Id1 at the mRNA and protein level . CD19 + B cells were cultured in X-vivo 15 over night before treatment with BMP-6 for the indicated time points. Total RNA was extracted and Id1 , Id2 or Id3 -expression was analysed by real-time RT-PCR; values are normalised to the expression level of PGK1 mRNA and expressed as relative quantification (2 -average ΔΔC T – relative to Id1 , Id2 or Id3 expression in the cell-line Ramos). One representative of three independent experiments is shown.

Article Snippet: Detection of the BMP-6-protein has been tried with the following antibodies: goat polyclonal anti-BMP-6 (Santa Cruz, San Diego, CA, USA), monoclonal mouse anti-BMP-6 and polyclonal goat anti-BMP-6 from R & D Systems (Abingdon, UK), and mouse monoclonal anti-BMP-6 (Chemicon International Inc, Temecula, CA, USA).

Techniques: Cell Culture, Expressing, Quantitative RT-PCR

Journal: BMC Immunology

Article Title: BMP-6 inhibits growth of mature human B cells; induction of Smad phosphorylation and upregulation of Id1

doi: 10.1186/1471-2172-6-9

Figure Lengend Snippet: Id protein expression was determined by western-blot analysis. CD19 + B cells were cultured in X-vivo 15 over night before treatment with BMP-6 for the indicated time points and cell lysates were prepared. One representative experiment of three is shown. HeLa cells were used as a positive control for Id1 protein detection.

Article Snippet: Detection of the BMP-6-protein has been tried with the following antibodies: goat polyclonal anti-BMP-6 (Santa Cruz, San Diego, CA, USA), monoclonal mouse anti-BMP-6 and polyclonal goat anti-BMP-6 from R & D Systems (Abingdon, UK), and mouse monoclonal anti-BMP-6 (Chemicon International Inc, Temecula, CA, USA).

Techniques: Expressing, Western Blot, Cell Culture, Positive Control

Journal: BMC Immunology

Article Title: BMP-6 inhibits growth of mature human B cells; induction of Smad phosphorylation and upregulation of Id1

doi: 10.1186/1471-2172-6-9

Figure Lengend Snippet: Anti-IgM rapidly upregulates BMP-6 mRNA expression in human B-cells . CD19-positive B cells were cultured in X-vivo 15 over night and stimulated with anti-IgM for the indicated time periods, before total RNA was extracted. The expression of BMP-6 mRNA by realtime RT-PCR; values are normalised to the expression level of PGK1 mRNA and expressed as relative quantification (2 -average ΔΔC T – relative to BMP-6 expression in the cell-line Ramos. One representative of five independent experiments is shown.

Article Snippet: Detection of the BMP-6-protein has been tried with the following antibodies: goat polyclonal anti-BMP-6 (Santa Cruz, San Diego, CA, USA), monoclonal mouse anti-BMP-6 and polyclonal goat anti-BMP-6 from R & D Systems (Abingdon, UK), and mouse monoclonal anti-BMP-6 (Chemicon International Inc, Temecula, CA, USA).

Techniques: Expressing, Cell Culture, Reverse Transcription Polymerase Chain Reaction

Journal: BMC Immunology

Article Title: BMP-6 inhibits growth of mature human B cells; induction of Smad phosphorylation and upregulation of Id1

doi: 10.1186/1471-2172-6-9

Figure Lengend Snippet: Fetal calf serum and human AB serum upregulates BMP-6 mRNA expression in human B-cells . CD19-positive B cells were cultured in X-vivo 15 over night and with fetal calf serum or human AB serum at the indicated dilutions for four hours, before total RNA was extracted. The expression of BMP-6 mRNA by realtime RT-PCR; values are normalised to the expression level of PGK1 mRNA and expressed as relative quantification (2 -average ΔΔC T – relative to BMP-6 expression in the cell-line Ramos. One representative of three independent experiments is shown.

Article Snippet: Detection of the BMP-6-protein has been tried with the following antibodies: goat polyclonal anti-BMP-6 (Santa Cruz, San Diego, CA, USA), monoclonal mouse anti-BMP-6 and polyclonal goat anti-BMP-6 from R & D Systems (Abingdon, UK), and mouse monoclonal anti-BMP-6 (Chemicon International Inc, Temecula, CA, USA).

Techniques: Expressing, Cell Culture, Reverse Transcription Polymerase Chain Reaction

Journal: BMC Immunology

Article Title: BMP-6 inhibits growth of mature human B cells; induction of Smad phosphorylation and upregulation of Id1

doi: 10.1186/1471-2172-6-9

Figure Lengend Snippet: BMP-6 inhibits proliferation of human B cells . A) CD19 + B cells were isolated from peripheral blood and stimulated in triplicates with anti-IgM (37.5 μg/ml) or anti-IgM and CD40 ligand (CD40L; 10 ng/ml) in the presence or absence of BMP-6 for 72 hours. DNA synthesis was measured by [3H]-thymidine incorporation for the last 18 hours. Data are given as relative proliferation obtained by normalizing the mean counts per minute (cpm) for each stimulation to the mean cpm obtained for anti-IgM stimulated cells ± SEM. (mean cpm = 25 352 for anti-IgM stimulated cells, *p ≤ 0.0002 (n = 8), **p ≤ 0.023 (n = 6). B) Dose dependent inhibition of BMP-6 of anti-IgM induced DNA-synthesis of CD19 + B cells (relative proliferation ± SEM, n = 3) and C) the Burkitt lymphoma cell line Ramos (relative proliferation ± SEM, n = 3). Ramos cells were cultured for 72 hours and [3H]-thymidine were added for the last 4 hours. D) Noggin (5 μg/ml) and BMP-6 (0,25 or 1 μg/ml) were preincubated for 1 h at 37°C and then added to the CD19 + B cells in the presence of anti-IgM(37.5 μg/ml). Cells were cultured for 72 h and DNA synthesis was measured by 3 H-thymidine incorporation. One representative of three separate experiments is shown (mean cpm ± SD of triplicates). E) Highly purified CD19 + CD27 - or CD19 + CD27 + cells were obtained by cell sorting of CD19 + cells and stimulated with anti-IgM in the presence or absence of BMP-6, as indicated for 72 hours. DNA synthesis was measuredby [3H]-thymidine incorporation. Data are given as relative proliferation obtained by normalizing the mean cpm for each stimulation to the mean cpm obtained for anti-IgM stimulated cells (mean cpm = 18 221 for CD19 + CD27 - naïve B cells, mean cpm = 8 930 for CD19 + CD27 + memory B cells, n = 5; * p ≤ 0,004, **p ≤ 0.001).

Article Snippet: Detection of the BMP-6-protein has been tried with the following antibodies: goat polyclonal anti-BMP-6 (Santa Cruz, San Diego, CA, USA), monoclonal mouse anti-BMP-6 and polyclonal goat anti-BMP-6 from R & D Systems (Abingdon, UK), and mouse monoclonal anti-BMP-6 (Chemicon International Inc, Temecula, CA, USA).

Techniques: Isolation, DNA Synthesis, Inhibition, Cell Culture, Purification, FACS

Journal: BMC Immunology

Article Title: BMP-6 inhibits growth of mature human B cells; induction of Smad phosphorylation and upregulation of Id1

doi: 10.1186/1471-2172-6-9

Figure Lengend Snippet: Ramos cells were cultured in the presence or absense of BMP-6 (1 μg/ml) for 48 h before analysis of cell death by PI staining. Data are shown as mean percentage PI + cells (± SEM, n = 3, p ≤ 0,001).

Article Snippet: Detection of the BMP-6-protein has been tried with the following antibodies: goat polyclonal anti-BMP-6 (Santa Cruz, San Diego, CA, USA), monoclonal mouse anti-BMP-6 and polyclonal goat anti-BMP-6 from R & D Systems (Abingdon, UK), and mouse monoclonal anti-BMP-6 (Chemicon International Inc, Temecula, CA, USA).

Techniques: Cell Culture, Staining

Journal: BMC Immunology

Article Title: BMP-6 inhibits growth of mature human B cells; induction of Smad phosphorylation and upregulation of Id1

doi: 10.1186/1471-2172-6-9

Figure Lengend Snippet: BMP-6 induces cell death in B cells . CD19 + CD27 - naïve B cells or CD19 + CD27 + memory B cells were cultured for 48 h with BMP-6 (1 μg/ml) with or without anti-IgM (37.5 μg/ml). Cell death (PI + cells) was then measured by flow cytometry analysis. Data are shown as mean percentage PI + cells from five independent donors (± SEM; p ≤ 0,003).

Article Snippet: Detection of the BMP-6-protein has been tried with the following antibodies: goat polyclonal anti-BMP-6 (Santa Cruz, San Diego, CA, USA), monoclonal mouse anti-BMP-6 and polyclonal goat anti-BMP-6 from R & D Systems (Abingdon, UK), and mouse monoclonal anti-BMP-6 (Chemicon International Inc, Temecula, CA, USA).

Techniques: Cell Culture, Flow Cytometry

Journal: BMC Immunology

Article Title: BMP-6 inhibits growth of mature human B cells; induction of Smad phosphorylation and upregulation of Id1

doi: 10.1186/1471-2172-6-9

Figure Lengend Snippet: BMP-6-induced phosphorylation of Smad1/5/8 in CD19 + B cells and Ramos, but not in HL60 cells . CD19 + B cells, or the cell lines Ramos or HL60

Article Snippet: Detection of the BMP-6-protein has been tried with the following antibodies: goat polyclonal anti-BMP-6 (Santa Cruz, San Diego, CA, USA), monoclonal mouse anti-BMP-6 and polyclonal goat anti-BMP-6 from R & D Systems (Abingdon, UK), and mouse monoclonal anti-BMP-6 (Chemicon International Inc, Temecula, CA, USA).

Techniques:

Journal: BMC Immunology

Article Title: BMP-6 inhibits growth of mature human B cells; induction of Smad phosphorylation and upregulation of Id1

doi: 10.1186/1471-2172-6-9

Figure Lengend Snippet: were cultured in X-vivo 15 over night before treatment with BMP-6 for 30 minutes, or for the indicated time points before total cell lysates were prepared. The amount of phosphorylated Smad 1/5/8 was determined by western-blot analysis. The membranes were reprobed for Smad1. One representative experiment of three is shown.

Article Snippet: Detection of the BMP-6-protein has been tried with the following antibodies: goat polyclonal anti-BMP-6 (Santa Cruz, San Diego, CA, USA), monoclonal mouse anti-BMP-6 and polyclonal goat anti-BMP-6 from R & D Systems (Abingdon, UK), and mouse monoclonal anti-BMP-6 (Chemicon International Inc, Temecula, CA, USA).

Techniques: Cell Culture, Western Blot

Journal: BMC Immunology

Article Title: BMP-6 inhibits growth of mature human B cells; induction of Smad phosphorylation and upregulation of Id1

doi: 10.1186/1471-2172-6-9

Figure Lengend Snippet: BMP-6 induced upregulation of Id1 at the mRNA and protein level . CD19 + B cells were cultured in X-vivo 15 over night before treatment with BMP-6 for the indicated time points. Total RNA was extracted and Id1 , Id2 or Id3 -expression was analysed by real-time RT-PCR; values are normalised to the expression level of PGK1 mRNA and expressed as relative quantification (2 -average ΔΔC T – relative to Id1 , Id2 or Id3 expression in the cell-line Ramos). One representative of three independent experiments is shown.

Article Snippet: Detection of the BMP-6-protein has been tried with the following antibodies: goat polyclonal anti-BMP-6 (Santa Cruz, San Diego, CA, USA), monoclonal mouse anti-BMP-6 and polyclonal goat anti-BMP-6 from R & D Systems (Abingdon, UK), and mouse monoclonal anti-BMP-6 (Chemicon International Inc, Temecula, CA, USA).

Techniques: Cell Culture, Expressing, Quantitative RT-PCR

Journal: BMC Immunology

Article Title: BMP-6 inhibits growth of mature human B cells; induction of Smad phosphorylation and upregulation of Id1

doi: 10.1186/1471-2172-6-9

Figure Lengend Snippet: Id protein expression was determined by western-blot analysis. CD19 + B cells were cultured in X-vivo 15 over night before treatment with BMP-6 for the indicated time points and cell lysates were prepared. One representative experiment of three is shown. HeLa cells were used as a positive control for Id1 protein detection.

Article Snippet: Detection of the BMP-6-protein has been tried with the following antibodies: goat polyclonal anti-BMP-6 (Santa Cruz, San Diego, CA, USA), monoclonal mouse anti-BMP-6 and polyclonal goat anti-BMP-6 from R & D Systems (Abingdon, UK), and mouse monoclonal anti-BMP-6 (Chemicon International Inc, Temecula, CA, USA).

Techniques: Expressing, Western Blot, Cell Culture, Positive Control

Journal: BMC Immunology

Article Title: BMP-6 inhibits growth of mature human B cells; induction of Smad phosphorylation and upregulation of Id1

doi: 10.1186/1471-2172-6-9

Figure Lengend Snippet: Anti-IgM rapidly upregulates BMP-6 mRNA expression in human B-cells . CD19-positive B cells were cultured in X-vivo 15 over night and stimulated with anti-IgM for the indicated time periods, before total RNA was extracted. The expression of BMP-6 mRNA by realtime RT-PCR; values are normalised to the expression level of PGK1 mRNA and expressed as relative quantification (2 -average ΔΔC T – relative to BMP-6 expression in the cell-line Ramos. One representative of five independent experiments is shown.

Article Snippet: Detection of the BMP-6-protein has been tried with the following antibodies: goat polyclonal anti-BMP-6 (Santa Cruz, San Diego, CA, USA), monoclonal mouse anti-BMP-6 and polyclonal goat anti-BMP-6 from R & D Systems (Abingdon, UK), and mouse monoclonal anti-BMP-6 (Chemicon International Inc, Temecula, CA, USA).

Techniques: Expressing, Cell Culture, Reverse Transcription Polymerase Chain Reaction

Journal: BMC Immunology

Article Title: BMP-6 inhibits growth of mature human B cells; induction of Smad phosphorylation and upregulation of Id1

doi: 10.1186/1471-2172-6-9

Figure Lengend Snippet: Fetal calf serum and human AB serum upregulates BMP-6 mRNA expression in human B-cells . CD19-positive B cells were cultured in X-vivo 15 over night and with fetal calf serum or human AB serum at the indicated dilutions for four hours, before total RNA was extracted. The expression of BMP-6 mRNA by realtime RT-PCR; values are normalised to the expression level of PGK1 mRNA and expressed as relative quantification (2 -average ΔΔC T – relative to BMP-6 expression in the cell-line Ramos. One representative of three independent experiments is shown.

Article Snippet: Detection of the BMP-6-protein has been tried with the following antibodies: goat polyclonal anti-BMP-6 (Santa Cruz, San Diego, CA, USA), monoclonal mouse anti-BMP-6 and polyclonal goat anti-BMP-6 from R & D Systems (Abingdon, UK), and mouse monoclonal anti-BMP-6 (Chemicon International Inc, Temecula, CA, USA).

Techniques: Expressing, Cell Culture, Reverse Transcription Polymerase Chain Reaction